Dissimilar responses of membrane potential (EM), permeability properties and respiration to cadmium and nickel in maize root cells

Fiala, R. and Luxová, M. and Čiamporová, M. and Repka, V. and Martinka, M. and Pavlovkin, J. (2015) Dissimilar responses of membrane potential (EM), permeability properties and respiration to cadmium and nickel in maize root cells. Cereal Research Communications, 43 (1). pp. 52-60. ISSN 0133-3720

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Abstract

The short-term treatment with Cd²⁺ and Ni²⁺ triggered transient depolarization of transplasma membrane potential (E_M) in the outer cortical root cells of two maize cultivars (cv. Premia and cv. Blitz), however, both metals changed the E_M in a quantitatively different way. The magnitude and duration of E_M depolarization were concentration dependent and were greater in the metal susceptible cv. Blitz. The highest E_M depolarization was recorded with simultaneous application of Cd²⁺ + Ni²⁺ in both maize cultivars. The E_M depolarization induced by Cd²⁺ or Cd²⁺ + Ni²⁺ but not Ni²⁺ alone was accompanied with a tremendous increase of membrane conductivity, but it was not accompanied with the effect of heavy metals (HM) on respiration. Simultaneous application of fusiccocin (FC) with Cd²⁺ or Cd²⁺ + Ni²⁺ during the E_M depolarization, inability of FC to stop the depolarization by FC-enhanced proton extrusion and rapid restoration of E_M, suggested a transient inhibition of the plasma membrane H⁺-ATPase by these toxic metals. Our data support the opinion that differences in the effects of the studied ions were not the result of their direct action on PM, but rather of their different influence on intracellular processes within root cells.

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