REAL

Seroreactivity to microbial components in Crohn's disease is associated with ileal involvement, noninflammatory disease behavior andNOD2/CARD15 genotype, but not with risk for surgery in a Hungarian cohort of IBD patients

Papp, Mária and Altorjay, István and Norman, Gary L. and Shums, Zakera and Palatka, Károly and Vitalis, Zsuzsanna and Földi, Ildikó and Lakos, Gabriella and Tumpek, Judit and Udvardy, Miklós L. and Hársfalvi, Jolán and Fischer, Simon and Lakatos, László and Kovács, Ágota and Bene, László and Molnár, Tamás and Tulassay, Zsolt and Miheller, Pál and Veres, Gábor and Papp, János and Lakatos, Péter László (2007) Seroreactivity to microbial components in Crohn's disease is associated with ileal involvement, noninflammatory disease behavior andNOD2/CARD15 genotype, but not with risk for surgery in a Hungarian cohort of IBD patients. Inflammatory Bowel Diseases, 13 (8). pp. 984-992. ISSN 1078-0998 (print), 1536-4844 (online)

[img] PDF
1080043.pdf
Restricted to Registered users only

Download (140kB) | Request a copy

Abstract

BACKGROUND: Antibodies directed against Saccharomyces cerevisiae (ASCA), perinuclear components of neutrophils (pANCA), and porin protein C of Escherichia coli (anti-OmpC) are reported to be associated with disease phenotype and may be of diagnostic importance in inflammatory bowel disease (IBD). Since limited data are available from Eastern Europe, we assessed the above antibodies in Hungarian IBD patients. METHODS: In all, 653 well-characterized, unrelated consecutive IBD patients (Crohn's disease [CD]: 558, m/f: 263/295, duration: 8.1 +/- 10.7 years; ulcerative colitis [UC]: 95, m/f: 44/51, duration: 8.9 +/- 9.8 years) and 100 healthy subjects were investigated. Sera were assayed for anti-Omp and ASCA by enzyme-linked immunosorbent assay (ELISA) and ANCA by indirect immunofluorescence assay (IIF). TLR4 and NOD2/CARD15 variants were tested by polymerase chain reaction/restriction fragment length polymorphism (PCR-RFLP). Detailed clinical phenotypes were determined by reviewing the medical charts. RESULTS: Anti-Omp, ASCA, and atypical pANCA antibodies were present in 31.2%, 59.3%, and 13.8% of CD, 24.2%, 13.7%, and 48.5% of UC patients, and in 20%, 16%, and 5.6% of controls, respectively. ASCA and anti-Omp positivity were associated with increased risk for CD (odds ratio [OR](ASCA) = 7.65, 95% confidence interval [CI]: 4.37-13.4; OR(Omp) = 1.81, 95% CI: 1.08-3.05). In a logistic regression analysis, anti-Omp and ASCA were independently associated with ileal and noninflammatory disease, but not with a risk for surgery or response to steroids or infliximab. A serology dosage effect was also observed. ASCA and anti-Omp antibodies were associated with NOD2/CARD15, in addition to a gene dosage effect. No associations were found in UC. CONCLUSIONS: Serological markers were useful in the differentiation between CD and UC in an Eastern European IBD cohort. Reactivity to microbial components was associated with disease phenotype and NOD2/CARD15 genotype, further supporting the role of altered microbial sensing in the pathogenesis of CD.

Item Type: Article
Subjects: R Medicine / orvostudomány > RC Internal medicine / belgyógyászat
Depositing User: Erika Bilicsi
Date Deposited: 03 Jan 2013 09:59
Last Modified: 03 Jan 2013 09:59
URI: http://real.mtak.hu/id/eprint/3744

Actions (login required)

Edit Item Edit Item