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Evans blue fluorescence permits the rapid visualization of non-intact cells in the perilesional rim of cold-injured rat brain

Rákos, Gabriella and Kis, Zsolt and Nagy, Dávid and Lür, György and Farkas, Tamás and Hortobágyi, Tibor and Vécsei, László and Toldi, József (2007) Evans blue fluorescence permits the rapid visualization of non-intact cells in the perilesional rim of cold-injured rat brain. Acta Neurobiologiae Experimentalis, 67 (2). pp. 149-154. ISSN 0065-1400

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Abstract

A focal cold lesion-induced injury, i.e., a model of focal vasogenic brain edema, enhances the permeability of the blood-brain barrier and cell membrane in the perilesional rim. However, non-intact cells can be detected, e.g. by markers of apoptosis, only hours or even days after the injury. The early membrane dysfunction allows extravasated serum proteins to enter the injured cells, which can be readily visualized if the plasma albumin was previously bound to fluorescent tracers, such as Evans Blue (EB). The aim of this study was to demonstrate injured cells that take up the EB/albumin conjugate in the perilesional rim. This tracer was administered 3.5 h after the induction of the injury and the animals were sacrificed 30 min later. With an excitation wavelength of 530-550 rim, the EB-positive cells emitted bright-red fluorescence at > 590 rim and were easy to count. No positive cells were observed in the controls. This method provides more information than the classical 2,3,5-triphenyltetrazolium chloride reaction, because it permits an assessment of the density and distribution of cells with non-intact cell membranes in the perilesional area following cerebrocortical injury.

Item Type: Article
Subjects: R Medicine / orvostudomány > RZ Other systems of medicine / orvostudomány egyéb területei
Depositing User: Erika Bilicsi
Date Deposited: 04 Jan 2013 11:34
Last Modified: 04 Jan 2013 11:34
URI: http://real.mtak.hu/id/eprint/3770

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