REAL

Hydroponic culturing upregulates sucrose and glutamine metabolism by enhancing their utilization via intermediates of aerobic pathway in wheat

Kaur, B. and Asthir, B. (2016) Hydroponic culturing upregulates sucrose and glutamine metabolism by enhancing their utilization via intermediates of aerobic pathway in wheat. Cereal Research Communications, 44 (3). pp. 381-392. ISSN 0133-3720

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Abstract

Two wheat genotypes were grown in hydroponic culture, containing 4 mM KNO<sub>3</sub>, NH<sub>4</sub>Cl and NH<sub>4</sub>NO<sub>3</sub>. Activities of N metabolizing enzymes, aminotransferases, carbohydrate and TCA cycle enzymes were analyzed along with protein, amino acid, N, sugar content and growth parameters in shoot and root. After 12 days, the size of shoot and root system decreased significantly when plants were supplied with NH<sub>4</sub>Cl as exclusive N source. Under NH<sub>4</sub>NO<sub>3</sub> growth parameters, N and carbon metabolism were elevated as compared to NH<sub>4</sub>Cl but less than KNO<sub>3</sub> source indicating inhibition of NH<sub>4</sub><sup>+</sup> toxicity by NO<sub>3</sub><sup>−</sup> uptake. Our results suggested that GDH, aminotransferases and PEPC play an important role in ammonium detoxification by its incorporation into amino acids. Thus, the morphologic differences among plants growing in NH<sub>4</sub><sup>+</sup> or NO<sub>3</sub><sup>−</sup> nutrition confirm the hypothesis that N source determines the growth habit of plant in wheat by modulating the endogenous levels of protein and sugar content.

Item Type: Article
Subjects: S Agriculture / mezőgazdaság > S1 Agriculture (General) / mezőgazdaság általában
Depositing User: Ágnes Sallai
Date Deposited: 20 Sep 2016 06:11
Last Modified: 30 Sep 2017 23:15
URI: http://real.mtak.hu/id/eprint/39647

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