Molecular detection of a putatively novel cyprinid herpesvirus in sichel (Pelecus cultratus) during a mass mortality event in Hungary

Doszpoly, Andor and Papp, Melitta and Deákné P., Petra and Glávits, Róbert and Ursu, Krisztina and Dán, Ádám (2015) Molecular detection of a putatively novel cyprinid herpesvirus in sichel (Pelecus cultratus) during a mass mortality event in Hungary. Archives of Virology, 160 (5). pp. 1279-1283. ISSN 0304-8608

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Abstract

In the early summer of 2014, mass mortality of Sichel (Pelecus cultratus) was observed in Lake Balaton, Hungary. Histological examination revealed degenerative changes within the tubular epithelium, mainly in the distal tubules and collecting ducts in the kidneys and multifocal vacuolisation in the brain stem and cerebellum. The routine molecular investigations showed the presence of the DNA of an unknown alloherpesvirus in some specimens. Subsequently, three genes were amplified and sequenced partially from the putative herpesviral genome (DNA polymerase, terminase, and helicase). Phylogenetic tree reconstruction, based on the concatenated sequence of these three conserved genes, implied that the virus undoubtedly belongs to the genus Cyprinivirus within the family Alloherpesviridae. The sequences of the Sichel herpesvirus differ markedly from those of the three known cypriniviruses (CyHV-1, CyHV-2 and CyHV-3); putatively representing the fourth virus species in the genus.

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