SHORT-TERM EFFECTS OF T-2 TOXIN OR DEOXYNIVALENOL ON LIPID PEROXIDATION AND THE GLUTATHIONE SYSTEM IN COMMON CARP

Pelyhe, Csilla and Kövesi, Benjámin and Zándoki, Erika and Kovács, Balázs and Szabó-Fodor, Judit and Mézes, Miklós and Balogh, Krisztián (2016) SHORT-TERM EFFECTS OF T-2 TOXIN OR DEOXYNIVALENOL ON LIPID PEROXIDATION AND THE GLUTATHIONE SYSTEM IN COMMON CARP. Acta Veterinaria Hungarica, 64 (4). pp. 449-466. ISSN 0236-6290 (In Press)

Preview

Text 449-466_004.2016.042_Pelyhe_64_4.pdf Download (209kB) | Preview

Abstract

The purpose of this study was to investigate the short-term effects of a sin-gle oral dose of T-2 and HT-2 toxin at 0.15, 0.33 and 1.82 mg kg–1 body weight, or deoxynivalenol (DON) and 15-acetyl-DON at 0.13, 0.31 and 1.75 mg kg–1 body weight in common carp. Conjugated dienes and trienes (the early markers of lipid peroxidation) were elevated in all DON-treated groups at the 16th hour, while thiobarbituric acid reactive substances (TBARS; termination marker) were increased at the highest dose of DON at the 16th and 24th hours. T-2 toxin did not cause changes in these parameters. Glutathione content and glutathione peroxi-dase activity showed higher levels at the 16th hour as the effect of both mycotox-ins. The expression of glutathione peroxidase (GPx4) genes (gpx4a and gpx4b) revealed a dual response. Downregulation was observed at the 8th hour, followed by an induction at the 16th hour, at the lowest dose of both mycotoxins. Higher doses revealed long-drawn emergence and an elevation was observed only at the 24th hour. However, at the lowest and highest doses of DON or T-2 toxin the changes in gene expression were delayed, which may be related to the low oxida-tive stress response, as suggested by the expression profiles of the nrf2, keap1, gpx4a and gpx4b genes.

Actions (login required)

Edit Item