Molecular analysis and MIRU-VNTR typing of Mycobacterium avium subsp. avium, ‘hominissuis’ and silvaticum strains of veterinary origin

Rónai, Zsuzsanna and Csivincsik, Ágnes and Dán, Ádám and Gyuranecz, Miklós (2016) Molecular analysis and MIRU-VNTR typing of Mycobacterium avium subsp. avium, ‘hominissuis’ and silvaticum strains of veterinary origin. INFECTION GENETICS AND EVOLUTION, 40. pp. 192-199. ISSN 1567-1348

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Abstract

Besides Mycobacterium avium subsp. paratuberculosis (MAP), M. avium subsp. avium (MAA), M. avium subsp. silvaticum (MAS), and ‘ M. avium subsp. hominissuis ’ (MAH) are equally important members of M. avium complex, with worldwide distribution and zoonotic potential. Genotypic discrimination is a prerequisite to epidemiologi- cal studies which can facilitate disease prevention through revealing infection sources and transmission routes. The primary aim of this study was to identify the genetic diversity within 135 MAA, 62 MAS, and 84 MAH strains isolated from wild and domestic mammals, reptiles and birds. Strains were tested for the presence of large sequence polymorphism LSP A 17 and were submitted to Mycobacte- rial interspersed repetitive units-variable-number tandem repeat (MIRU-VNTR) analysis at 8 loci, including MIRU1, 2, 3, and 4, VNTR25, 32, and 259, and MATR9. In 12 strains hsp 65 sequence code type was also determined. LSP A 17 was present only in 19.9% of the strains. All LSP A 17 positive strains belonged to subspecies MAH. The discriminatory power of the MIRU-VNTR loci set used reached 0.9228. Altogether 54 different genotypes were detected. Within MAH, MAA, and MAS strains 33, 16, and 5 different genotypes were observed. The described genotypes were not restricted to geographic regions or host species, but proved to be subspecies speci fi c. Ourknowledge about MAS islimited due toisolation and identi fi cation dif fi culties.Thisisthe fi rst studyincluding a large number of MAS fi eld strains. Our results demonstrate the high diversity of MAH and MAA strains and the relative uniformity of MAS strains.

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