Composition of endophytic fungal community associated with leaves of maize cultivated in south Brazilian field

Szilagyi-Zecchin, Vivian J. and Adamoski, Douglas and Gomes, Renata Rodrigues and Hungria, Mariangela and Ikeda, Angela C. and Kava-Cordeiro, Vanessa and Glienke, Chirlei and Galli-Terasawa, Lygia V. (2016) Composition of endophytic fungal community associated with leaves of maize cultivated in south Brazilian field. Acta Microbiologica et Immunologica Hungarica, 63 (4). pp. 449-466. ISSN 1217-8950

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Abstract

The objective of this study was to conduct a survey about fungi associated with leaves from two different maize plant lineages and to analyze their microbiota diversity. Isolated fungi were identified by morphological analysis and molecular taxonomy was performed using ITS1-5.8S-ITS2 rDNA. About 27 fungi morphotypes were obtained, 15 of them were from the first maize lineage. About 86.7% of the individuals belonged to the Dothideomycetes class (Phoma sorghina, Epicocum nigrum, Cladosporium sp., Bipolaris zeicola, and Alternaria alternata complex) and 13.3% to the Sordariomycetes class (Diaporthe/Phomopsis sp. and Nigrospora sp.). This ratio was opposite in the other maize lineage with 25.0% of Dothideomycetes (E. nigrum and Pleosporales) and 75.0% of Sordariomycetes (Gibberella fujikuroi complex, Fusarium graminearum complex, Diaporthe/Phomopsis sp., and Nigrospora sp.). By concerning the analyses of morphological characteristics and molecular phylogeny, this study intended to identify the groups of saprophytic, phytopathogenic, and mycotoxin fungi, which differently co-inhabit leaf tissue of maize plants in both tested lineages.

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