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Elevated basal cytosolic calcium of endothelial cells influences the post-surgical outcome in diabetic CTS

Bin-Jaliah, IM and Shariff, MEA and Nayar, PS and Chandramoorthy, HC (2016) Elevated basal cytosolic calcium of endothelial cells influences the post-surgical outcome in diabetic CTS. Physiology International, 103 (4). pp. 469-480. ISSN 2498-602X

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Abstract

<sec sec-type="background"> Background Type 2 diabetes mellitus (T2DM)-induced neuropathy and ischemia-reperfusion post-surgery prolong carpal tunnel syndrome (CTS) pathology, but the effect of T2DM on the prognostic outcome of carpal tunnel (CT) release surgery needs to be investigated.<sec sec-type="materials and methods"> Materials and methods A total of 64 individuals with CTS underwent CT release surgery. HbA1c levels identified their diabetic status. The individual prognostic outcomes were measured by nerve conduction velocity (NCV), amplitude, and latency. Measurement of [Ca<sup>2+</sup>]<sub>c</sub> and reactive oxygen species (ROS) from isolated endothelial cells (ECs) revealed the oxidative burden of the normal and diabetic CTS phenotypes. Results CTS individuals with HbA1c > 7 showed decreased NCV (≈22 m/s) and amplitude (≈4.2 mV) with increased latency (≈6 ms), compared to groups with HbA1c ≤ 7. Further to CT release surgery, the reversal of the nerve conduction to normalcy was greatly influenced by the diabetic profile of the individuals. Our results showed elevated basal [Ca<sup>2+</sup>]<sub>c</sub> and corresponding high cytosolic ROS in the ECs isolated from individuals with HbA1c > 7 compared to the diabetic and healthy control groups.<sec sec-type="conclusion"> Conclusion The individuals with diabetic index showed suboptimal neuronal performance pre- and post-CT release surgery. Oxidative stress mediated by high [Ca<sup>2+</sup>]<sub>c</sub> and ROS of ECs dissipates to adjoining cells worsening the pathology of the untreated CTS.

Item Type: Article
Subjects: R Medicine / orvostudomány > R1 Medicine (General) / orvostudomány általában
Depositing User: Ágnes Sallai
Date Deposited: 26 Jan 2017 08:54
Last Modified: 31 Dec 2017 00:17
URI: http://real.mtak.hu/id/eprint/46427

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