The role of hydrogen sulfide in homocysteine-induced cardiodynamic effects and oxidative stress markers in the isolated rat heart

Stojanovic, M and Zivkovic, V and Srejovic, I and Jakovljevic, V and Jeremic, N and Djuric, D (2016) The role of hydrogen sulfide in homocysteine-induced cardiodynamic effects and oxidative stress markers in the isolated rat heart. Physiology International, 103 (4). pp. 428-438. ISSN 2498-602X

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Abstract

This study aimed to assess the role of H₂S in homocysteine-induced cardiodynamic effects in the isolated rat heart. The hearts were retrogradely perfused according to the Langendorff technique. The maximum and minimum rates of pressure in the left ventricle (dp/dt max, dp/dt min), systolic and diastolic left ventricular pressures (SLVP, DLVP), heart rate (HR), and coronary flow (CF) were measured. A spectrophotometrical method was used to measure the following oxidative stress markers: index of lipid peroxidation (thiobarbituric acid reactive substances, TBARS), nitrite level (NO₂⁻), superoxide anion radicals (O₂^•−), and hydrogen peroxide (H₂O₂) concentrations. The administration of 10 µmol/l DL-homocysteine (DL-Hcy) alone decreased dp/dt max, SLVP, and CF but did not change any oxidative stress parameters. The administration of 10 µmol/l DL-propargylglycine (DL-PAG) decreased all cardiodynamic parameters and increased the concentration of O₂^•−. The co-administration of DL-Hcy and DL-PAG induced a significant decrease in all estimated cardiodynamic parameters and decreased the concentration of NO₂⁻ and O₂^•− but increased the levels of TBARS and H₂O₂. Homocysteine shows a lower pro-oxidative effect in the presence of hydrogen sulfide (H₂S), which indicates a potential anti-oxidative capacity of H₂S.

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