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Daidzein affects steroidogenesis and oestrogen receptor expression in medium ovarian follicles of pigs

Nynca, Anna and Słonina, Dominika and Jablońska, Olga and Kamińska, Barbara and Ciereszko, Renata (2013) Daidzein affects steroidogenesis and oestrogen receptor expression in medium ovarian follicles of pigs. Acta Veterinaria Hungarica, 61 (1). pp. 85-98. ISSN 0236-6290

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Abstract

Daidzein, a phytoestrogen present in soybean products used in swine feed, has been demonstrated to affect both reproductive and endocrine functions. The aims of this study were to examine the in vitro effects of daidzein on (1) progesterone (P4) and oestradiol (E2) secretion by porcine luteinised granulosa cells harvested from medium follicles, and (2) the mRNA and protein expression of oestrogen receptors α and β (ERα and ERβ) in these cells. The influence of E2 on P4 secretion and ERα and ERβ expression in the granulosa cells of pigs was also investigated. It was found that daidzein inhibited progesterone secretion by luteinised granulosa cells isolated from medium follicles. In contrast, E2 did not affect progesterone production by these cells. Moreover, daidzein did not alter the granulosal secretion of E2. Both daidzein and E2 decreased mRNA expression of ERα in the cells examined. The expression of ERβ mRNA was not affected by daidzein but was inhibited by E2. ERα protein was not detected while ERβ protein was found in the nuclei of the cells. Daidzein and E2 upregulated the expression of ERβ protein in the cells. In summary, the phytoestrogen daidzein directly affected the porcine ovary by inhibiting progesterone production and increasing ERβ protein expression. Daidzein-induced changes in follicular steroidogenesis and granulosal sensitivity to oestrogens may disturb reproductive processes in pigs.

Item Type: Article
Subjects: S Agriculture / mezőgazdaság > SV Veterinary science / állatorvostudomány
Depositing User: xKatalin xBarta
Date Deposited: 07 Feb 2017 12:24
Last Modified: 07 Feb 2017 12:24
URI: http://real.mtak.hu/id/eprint/47546

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