Use of RAPD-PCR, plasmid profiling, class 1 integron analysis, and antimicrobial resistance for molecular characterisation of Salmonella strains isolated from Turkey

Taşkale, N. and Akçelik, M. (2012) Use of RAPD-PCR, plasmid profiling, class 1 integron analysis, and antimicrobial resistance for molecular characterisation of Salmonella strains isolated from Turkey. Acta Alimentaria, 41 (1). pp. 56-66. ISSN 0139-3006

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Abstract

Forty-one Salmonella strains, isolated from food samples collected from different parts of Turkey were characterized by various biochemical and genotypic tests. Among 41 isolates, 10 strains harboured at least one plasmid, sizes ranging from 3 kb to 400 kb. The antibiotic resistance profiles of Salmonella isolates were studied against 16 commonly used antibiotics. The highest level of resistance was observed against sulfonamide and neomycin, respectively. Moreover, resistance to multiple antibiotics (5) was also observed among 59% of isolates. Each isolate was also screened for the presence of class 1 integrons and nearly 88% of strains contained class 1 integron with different variable regions (VRs). Further examination of the Salmonella isolates was carried out by testing their clonal relation by RAPD-PCR analyses. With the amplification of primer P1254 the isolates classified into 15 groups and with primer P1283, they classified into 8 groups, on the basis of statistical analyses.

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