Influence of the culture medium composition on the exopolysaccharides synthesis by Streptococcus sp. IL5 strain

Vamanu, E. and Vamanu, A. and Pelinescu, D. and Niţă, S. and Rusu, N. and Popa, N. (2012) Influence of the culture medium composition on the exopolysaccharides synthesis by Streptococcus sp. IL5 strain. Acta Alimentaria, 41 (1). pp. 118-125. ISSN 0139-3006

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Abstract

The synthesis of exopolysaccharides was determined for the Streptococcus sp. IL5 strain in MRS and M17 medium, with carbon sources such as glucose, lactose and sucrose. According to the obtained results, by using glucose as carbon source, the highest quantity of exopolysaccharides was obtained, namely of 400 mg l⁻¹. The influence of various glucose concentrations (5, 10, 15, 20%) on exopolysaccharide production was tested. It resulted in that, at a concentration of 15% glucose, a maximum quantity of synthesized exopolysaccharides was obtained. The influence of temperature and of pH on the exopolysaccharide synthesis capacity of the strain was tested as well. From the tests the temperature of 37 °C and pH 7 proved to be the ideal parameters for obtaining a maximum exopolysaccharides quantity. Based on chromatographic methods, the content of synthesized polysaccharide was determined, consisting of 31.87% anhydrous glucose with a degree of polymerization of 36.7%.

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