Sex-related alterations of aminopyrine metabolism by standardised extract of Eurycoma Longifolia (TAF-273)

Purwantiningsih, and Hussin, A. and Chana, K. (2012) Sex-related alterations of aminopyrine metabolism by standardised extract of Eurycoma Longifolia (TAF-273). Acta Alimentaria, 41 (3). pp. 316-326. ISSN 0139-3006

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Abstract

Previous study showed that the standardised 50% aqueous-methanol extract of Eurycoma longifolia (TAF-273) affected phase 1 aminopyrine metabolism especially in adult male and female rat hepatocytes. The present study evaluated the molecular mechanism of TAF-273 on phase 1 aminopyrine metabolism in adult male and female rat hepatocytes by using ten cellular stimulants/inhibitors. The study suggested that the effect of TAF-273 on hepatic phase 1 aminopyrine metabolism in adult male rats was probably mediated through the inhibition of tyrosine kinases and through the activation of G-protein, protein kinase G, protein kinase A in the cAMP pathway, calmodulin and protein kinase C. On the contrary, the extract affected adult female rat hepatocytes, only through the activation of G-protein, protein kinase G, protein kinase A in the cAMP pathway and protein kinase C. The effect of TAF-273 on aminopyrine metabolism in the presence of trifluoperazine, phorbol myristate acetate and genistein also exhibited significant difference (P<0.05) between male and female rats. The rate of aminopyrine metabolism was higher in male rats than in female rats and the effect of TAF-273 on aminopyrine metabolism at the molecular level was different in male and female rats on the activation of calmodulin, protein kinase C and tyrosine kinases.

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