REAL

Effect of cadmium injected in ovo on hatching results and the activity of plasma hydrolytic enzymes in newly hatched chicks

Dżugan, Małgorzata and Lis, Marcin and Droba, Maria and Niedziółka, Jerzy (2011) Effect of cadmium injected in ovo on hatching results and the activity of plasma hydrolytic enzymes in newly hatched chicks. Acta Veterinaria Hungarica, 59 (3). pp. 337-347. ISSN 0236-6290

[img] Text
avet.2011.020.pdf
Restricted to Repository staff only until 30 September 2031.

Download (221kB)

Abstract

The aim of the study was to determine the toxicity of cadmium ions in chick embryos, using plasma hydrolytic enzyme as its biomarker. Hatching eggs (n = 300) from Ross 308 broilers were incubated under standard conditions. On day 4 of incubation, 50 μl of saline solution, containing Cd ions at a concentration from 0 (control group) to 24 μg, was injected in ovo into the egg albumen. The results indicate that the administration of cadmium at doses exceeding 1 μg/egg caused a gradual decrease in hatchability, with an LD<sub>50</sub> of 3.9 μg/egg. The greatest differences between the groups in the enzymatic activities studied were found for N-acetyl-β-D-glucosaminidase (NAG), β-D-mannosidase (β-MAN) and arylsulphatase (ARYL). Compared to the control group, in the blood serum of chicks from the groups receiving 3, 6 and 12 μg Cd/egg the NAG activity increased by 79, 108 and 54% and β-MAN activity by 33, 119 and 108%, respectively. Exposure to cadmium at a dose of 1 to 6 μg per egg caused an about 60% increase in ARYL activity while a dose of 12 μg decreased the activity by about 35% below the level observed in the control group. These findings show that cadmium has a similar toxicity mechanism in mammals and birds, which opens the possibility of using NAG activity as a biomarker of the cytotoxic effect of cadmium in birds.

Item Type: Article
Subjects: S Agriculture / mezőgazdaság > SV Veterinary science / állatorvostudomány
Depositing User: xKatalin xBarta
Date Deposited: 09 Feb 2017 13:43
Last Modified: 09 Feb 2017 13:43
URI: http://real.mtak.hu/id/eprint/47986

Actions (login required)

Edit Item Edit Item