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In vitro adhesion of lactic acid bacteria and bifidobacteria to Caco-2P and IEC-18 cells

Szekér, K. and Beczner, J. and Halász, A. and Mayer, Á. and Rezessy-Szabó, J.M. and Gálfi, P. (2005) In vitro adhesion of lactic acid bacteria and bifidobacteria to Caco-2P and IEC-18 cells. Acta Alimentaria, 34 (1). pp. 91-99. ISSN 0139-3006

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Abstract

The adhesion of twenty-six Lactobacillusstrains to two intestinal cell lines (Caco-2P and IEC-18) and 21 Bifidobacteriumstrains to Caco-2P cells was investigated. Non-specific adherence was determined on the surface of culture plates. The effect of short chain fatty acids (SCFA) on epithelial cells, and bacterial adhesion were investigated by Na-n-butyrate treatment. The adherence of LAB and bifidobacteria greatly varied in a strain-dependent manner. The adherence of LAB was better to IEC-18 cells than to Caco-2P cells, and bifidobacteria adhered better to Caco-2P cells than the LAB. Some strains adhered well or even better to the background than to the cells, which queries the specificity of adhesion of these strains. Na-n-butyrate treatment stimulated the differentiation of IEC-18 cells and therefore increased the number of adherent bacteria, probably because only the cell surface increased not the number of epitopes.

Item Type: Article
Subjects: Q Science / természettudomány > QD Chemistry / kémia > QD01 Analytical chemistry / analitikai kémia
Depositing User: xKatalin xBarta
Date Deposited: 14 Feb 2017 14:15
Last Modified: 14 Feb 2017 14:15
URI: http://real.mtak.hu/id/eprint/48844

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