Binding of mycoplasmas to solid phase adsorbents

Szathmáry, Susan and Rajapakse, Nandani and Székely, Ibolya and Pitlik, E. and Bíró, Judit and Erdei, Noémi and Stipkovits, L. (2005) Binding of mycoplasmas to solid phase adsorbents. Acta Veterinaria Hungarica, 53 (3). pp. 299-307. ISSN 0236-6290

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Abstract

The capture of mycoplasmas (M. hominis, M. buccale, M. fermentans, M. bovis, M. synoviae, M. gallisepticum and M. arthritidis) based on lipid structures and adhesion molecules present in the mycoplasmal membrane was tested using different chromatographic resins (ActiClean Etox, ClarEtox, Heparin-Actigel, Sulfated Hiflow and SulfEtox). All of the resins efficiently reduced mycoplasma concentrations in Phosphate Buffered Saline (PBS) and in Fetal Bovine Serum (FBS) by 3-8 logs in a few minutes. This technology could be used for removing mycoplasmas from tissue culture components such as serum, and for concentrating mycoplasmas in vaccine production.

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