Use of conductimetric technique for data capture in predictive microbiology

Szigeti, E. and Farkas, J. (2000) Use of conductimetric technique for data capture in predictive microbiology. Acta Alimentaria, 29 (3). pp. 307-314. ISSN 0139-3006

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Abstract

Conductimetry as an alternative data capture method for following microbial growth has a great potential as a research tool of predictive microbiology. In spite of this fact there is only a limited number of applications using conductimetric data for model generation. In this study the growth of single strains of Listeria monocytogenes and Lactococcus lactis was tested in 5 media using a RABITinstrument. The goal of the work was to find selective growth media for Listeria and Lactococcus, respectively, in order to study their interaction in mixed-culture using the conductimetric technique. Whitley Anaerobic broth, Whitley Impedance broth and modified Whitely Impedance broth (Whitley Impedance broth + Chloramphenicol 7 mg l Ś1) were not suitable for following selectively the growth of Lactococcus lactis or Listeria monocytogenes in a mixed culture of the two bacteria. BiMedia 630 A for Lactococcus lactis and Bimedia 403 A for Listeria monocytogenes satisfied the demands raised by conductance measurement. Linear correlations were established between the graphically estimated TTD values of the conductance curves and the logarithmic numbers of colony forming units (CFU). The correlations were very strong in each case (determination coefficients (R 2) of the linear regression were higher than 0.98 at both medium-strain combinations). However, in BiMedia 630 Listeria monocytogenes was capable of slow growth, therefore, this medium would be feasible for studying microbial interactions if only low concentrations of Listeria (less than 10 6 CFU ml Ś1) were present in the mixed culture.

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