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Rapid detection of Salmonella in food by redox-potential measurement based method combined with real-time PCR

Erdősi, O. and Szakmár, K. and Reichart, O. and Szili, Zs. and László, N. and Balogh, Z. and Székely Körmöczy, P. and Laczay, P. (2014) Rapid detection of Salmonella in food by redox-potential measurement based method combined with real-time PCR. Acta Alimentaria, 43 (4). pp. 660-667. ISSN 0139-3006

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Abstract

The classical ISO (2002) standard as reference method and the combination of redox potential measurement with real-time PCR technique were applied to detect Salmonella in milk, egg, broiler meat, and artificially contaminated egg samples. Food samples of 25 g were homogenized in 225 ml of RVS broth to prepare the basic suspension of the comparative tests. In the combined method the redox potential measurement technique serves as the selective enrichment system of the real-time PCR equipment. The reliable screening of Salmonella-free, negative samples by the redox potential measurement technique needed only 24 h. These negative samples determined by the PCR and the classical standard method in all cases proved to be negative as well. In case of positive redox result the Salmonella from the enriched suspension of the redox test-cell was identified by real-time PCR in 3 hours, instead of the conventional biochemical identification. Comparing our protocol to the ISO (2002) standard method, the total detection time of Salmonella presence/absence was less than 24 h contrary to the 114 h of the conventional method.

Item Type: Article
Subjects: Q Science / természettudomány > QD Chemistry / kémia > QD01 Analytical chemistry / analitikai kémia
Depositing User: Erika Bilicsi
Date Deposited: 28 Feb 2017 19:56
Last Modified: 04 Apr 2023 12:35
URI: http://real.mtak.hu/id/eprint/49896

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