Genoprotectivity of methanol and ethanol extracted leaf sap of Trigonella foenum-graecum in Allium cepa root assay

Mekki, Laila (2014) Genoprotectivity of methanol and ethanol extracted leaf sap of Trigonella foenum-graecum in Allium cepa root assay. Acta Biologica Hungarica, 65 (1). pp. 85-95. ISSN 0236-5383

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Abstract

Fenugreek (Trigonella foenum-graecum) of Fabacecae family is widely distributed throughout the world and used as an old medicinal plant and traditional food. The present study deals with the investigation of the anti-genotoxic potential of methanol (MTG) and ethanol (ETG) extracted leaf sap of fenugreek on Allium cepa root tip cells, which were treated with cadmium sulfate (CdSO₄). Three types of treatments were applied. First, roots were treated with different concentrations of methanolic and ethanolic extracts (0.1%, 0.5% and 1%) separately for 3 h each, followed by CdSO₄ treatment (at 250 ppm, for 3 h). Second, roots were first treated with CdSO₄ followed by extracts treatment. Third, root tips were treated with CdSO₄ with extracts treatments at the same time. For controls, roots with CdSO₄ (250 ppm) and distilled water served as positive and negative control, respectively. The results showed that the methanol and ethanol extracts of fenugreek modulated the genotoxic and clastogenic aberrations, which were induced by CdSO₄. The protection activity of MTG (1%) was 50% in the first treatment, 70% in the second treatment and 82% in the third treatment and 61%, 68% and 88% of ETG (1%), respectively. DNA rearrangements were also observed by revealing new RAPD bands in the total DNS samples isolated from Allium roots after treatmenst.

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