Rapid genotyping of genetically modified laboratory animals from whole blood samples without DNA preparation

Horváth, Á. and Sántha, P. and Horváth, V. and Török, Nóra and Nagy, I. and Jancsó, G. and Vágvölgyi, Cs. and Somogyvári, F. (2013) Rapid genotyping of genetically modified laboratory animals from whole blood samples without DNA preparation. Acta Biologica Hungarica, 64 (2). pp. 262-265. ISSN 0236-5383

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Abstract

A new, rapid method is described which permits the genotyping of genetically modified animals from a microlitre volume of whole blood samples via one step polymerase chain reaction amplification. The major advantage of the presented method is the exclusion of a DNA preparation step, which significantly reduces the time expenditure and work load of the genetic testing. Pilot studies indicate, that this method is efficient and applicable also on tissue biopsies and larger amount of blood providing a rapid and reliable new technique over conventional genotyping approaches.

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