Expression patterns of Th1/Th2 transcription factors in patients with guttate psoriasis

Vanaki, Elaheh and Ataei, Mitra and Sanati, Mohammad and Mansouri, Parvine and Mahmoudi, Mahmoud and Zarei, Feresteh and Jadali, Zohreh (2013) Expression patterns of Th1/Th2 transcription factors in patients with guttate psoriasis. Acta Microbiologica et Immunologica Hungarica, 60 (2). pp. 163-174. ISSN 1217-8950

[img] Text
Restricted to Repository staff only until 30 June 2033.

Download (79kB)


Many lines of evidence propose that psoriasis is a T cell-mediated disease where T cell activation is followed by secretion of inflammatory cytokines. To elucidate the functional state of T cells in guttate psoriasis, we analysed mRNA expression levels of T-bet and GATA-3 for Th1 and Th2 differentiation, respectively together with Th1 (IFN-γ) and Th2 (IL-4) cytokine mRNA expression. Relative quantification of T-bet, GATA-3, IFN-γ and Th2, and IL-4 transcripts in peripheral blood leukocytes (PBL) was conducted by real-time reverse transcriptase PCR (RT-PCR). Serum levels of IFN-γ and Th2 and IL-4 were also determined by ELISA. GATA-3 and IL-4 mRNA expression levels were lower in psoriatic patients as compared to normal healthy controls. The expression levels of T-bet and IFN-γ and Th2 genes were relatively similar in the patients and controls. In addition, a marked decrease in plasma IL-4 levels was observed in the psoriasis group, while no differences were observed with regard to levels of IFN-γ and Th2 between patients and normal subjects. Furthermore, a clear correlation between decreased IL-4 mRNA expression and IL-4 (P < 0.05) was revealed. These results suggested that altered balance between Th1 and Th2 cells transcription factor genes and they products may be implicated in the pathogenesis of psoriasis.

Item Type: Article
Subjects: Q Science / természettudomány > QR Microbiology / mikrobiológia
Depositing User: Ágnes Sallai
Date Deposited: 20 Apr 2017 07:52
Last Modified: 20 Apr 2017 07:52

Actions (login required)

Edit Item Edit Item