REAL

Molecular characterization of vancomycin-resistant enterococci in Serbia: Intensive care unit as the source

Jovanović, Milica and Milošević, Branko and Dulović, Olga and Pelemiš, Mijomir and Stevanović, Goran and Tošić, Tanja and Stošović, Branka and Zervos, M.J. (2013) Molecular characterization of vancomycin-resistant enterococci in Serbia: Intensive care unit as the source. Acta Microbiologica et Immunologica Hungarica, 60 (4). pp. 433-446. ISSN 1217-8950

[img] Text
amicr.60.2013.4.5.pdf
Restricted to Repository staff only until 31 December 2033.

Download (73kB)

Abstract

The purpose of this study was to evaluate the molecular relatedness of clinical isolates of vancomycin-resistant enterococci (VRE) collected from patients of the Clinic for Infectious and Tropical Diseases in Belgrade. Among 40 isolates available for the investigation, 36 were identified as Enterococcus faecium, whereas 2 were Enterococcus faecalis and Enterococcus raffinosus, respectively. Pulsed-field gel electrophoresis (PFGE) typing revealed 21 strain types, comprising 7 clusters which contained at least two isolates and 14 unique PFGE patterns. Although we searched for pathogenicity factor genes (gelE, cylB, asa1, efaAfs, esp, cpd, cob) in representatives of all macro-restriction patterns, they have been confirmed in only one clone of E. faecalis. Genes esp and hyl, commonly found in E. faecium, were yilded in 10 macro-restriction patterns of this species, and their presence could not be ascribed to clonally related strains (p = 0.05). All VRE isolates were multiresistant and positive for vanA gene. Twenty strains of VRE and 6 clusters obtained from Intensive care unit (ICU) are proof of intensive transmission of these microorganisms at this department. The results of this study suggest wide genotypic variability among the clinical VRE isolates, but also intrahospital dissemination of some of them.

Item Type: Article
Subjects: Q Science / természettudomány > QR Microbiology / mikrobiológia
Depositing User: Ágnes Sallai
Date Deposited: 20 Apr 2017 08:14
Last Modified: 20 Apr 2017 08:14
URI: http://real.mtak.hu/id/eprint/51572

Actions (login required)

Edit Item Edit Item