Immunodetection, electrophoresis and amino acid composition of alcohol soluble proteins extracted from grains of selected varieties of pseudocereals, legumes, oat, maize and rice

Mickowska, B. and Socha, P. and Urminská, D. and Cieślik, E. (2013) Immunodetection, electrophoresis and amino acid composition of alcohol soluble proteins extracted from grains of selected varieties of pseudocereals, legumes, oat, maize and rice. Cereal Research Communications, 41 (1). pp. 160-169. ISSN 0133-3720

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Abstract

Prolamins are alcohol-soluble fraction of cereal proteins involved in immunological response of patients with celiac disease. The aim of this study was to analyse the similar protein complex of selected varieties of cereal, pseudocereal and legume grains by comparison of protein fractions, amino acids composition and SDS-PAGE electrophoresis. The immunoreactivity was tested by Western blot and ELISA methods. ELISA analysis recognizes celiac active epitopes in wheat gliadin (which is reference protein in determination of celiac activity), and also corresponding epitopes in other grain proteins responsible for immunological response of patients with celiac disease. Estimated quantity of celiac active sequences (calculated as gliadin content) below 20 ppm was found in species of amaranth, buckwheat and millet, as well as rice, maize, chickpea and chickling vetch. Immunological reaction with polyclonal antibody was negative for all crops, except oat, maize, millet and foxtail millet.

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