Application of a rapid electrophoresis technique analysing the glutenin subunit composition of wheat genotypes

Kovács, A. and Rakszegi, M. and Láng, L. and Ma, W. and Békés, F. and Bedő, Z. (2013) Application of a rapid electrophoresis technique analysing the glutenin subunit composition of wheat genotypes. Cereal Research Communications, 41 (3). pp. 468-481. ISSN 0133-3720

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Abstract

The characterization of the old Hungarian varieties and landraces is an important part of Hungarian cereal research and breeding. Analysis of these germplasms with the most up-to-date methodologies results a broad scale of diversity of glutenin alleles, which proves their genetic heterogenicity. Exploitation of this attribute is an untapped possibility for developing modern varieties in our breeding programs. The previous research work revealed this diversity by SDS-PAGE analysis and MALDI-TOF technology. The powerful tool, the high throughput lab-on-a chip technique can facilitate the effectiveness of this function and decreases the cost of the analysis. This study demonstrates the application of this technique for analysing the old varieties. The allelic composition and their effects on bread making quality concluded by means of functional analysis.

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