Validation of allele specific primers for identification of Rht genes among Indian bread wheat varieties

Nalini, E. and Bhagwat, S. G. and Jawali, N. (2005) Validation of allele specific primers for identification of Rht genes among Indian bread wheat varieties. Cereal Research Communications, 33 (2-3). pp. 439-446. ISSN 0133-3720

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Abstract

The dwarfing genes Rht-B1b and Rht-D1b were incorporated into Indian bread wheats during the “green revolution”. This led to a dramatic increase in yield as well as production of wheat. Although the presence of dwarfing genes is important, their status among the released wheat varieties is generally not known. We present an analysis of the two dwarfing genes using the PCR based “perfect markers” in bread wheat ( Triticum aestivum L. ) varieties, for the first time in 45 Indian, and some non-Indian wheats. Most of the varieties analyzed had either Rht-B1b or Rht-D1b or both. The results obtained for the varieties with known Rht status validated the perfect markers. Analysis of a F ₂ population segregating for the dwarfing genes also confirmed the utility of these markers in genetic analysis. Analysis among some of the varieties for the GA response phenotype is also presented. The results showed that the “perfect markers” are robust enough to be used among Indian varieties.

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