The effect of liquid and solid medium on production of winter triticale (× Triticosecale Wittm.) anther-derived embryos and plants

Ponitka, A. and Ślusarkiewicz-Jarzina, A. (2007) The effect of liquid and solid medium on production of winter triticale (× Triticosecale Wittm.) anther-derived embryos and plants. Cereal Research Communications, 35 (1). pp. 15-22. ISSN 0133-3720

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Abstract

The efficiency of anther culture induction in solid and liquid medium C17 was compared for nine triticale genotypes. For all genotypes androgenic embryos and green plants efficiency was higher on liquid medium. On liquid medium 22.2–135.9 and on solid medium 27.5–121.3 androgenic embryos/100 anthers were produced. The embryos were transferred to a regeneration medium 190-2 and also a higher number of green plants (ranging from 3.0 to 31.4/100 anthers) were obtained in liquid culture compared with the yield of plants (1.6–21.0) on solid medium.

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