Diversity in seed storage proteins in substituted hexaploid triticale cultivars (× Triticosecale Wittmack)

Kozub, N. A. and Xynias, I. N. and Sozinov, I. A. (2007) Diversity in seed storage proteins in substituted hexaploid triticale cultivars (× Triticosecale Wittmack). Cereal Research Communications, 35 (3). pp. 1469-1476. ISSN 0133-3720

Text crc.35.2007.3.11.pdf Restricted to Repository staff only until 30 September 2027. Download (450kB)

Abstract

This study was undertaken to investigate the allelic diversity in seed storage proteins in 11 substituted hexaploid triticale cultivars (all spring). These cultivars were developed at the Cereal Institute of Thessaloniki, Hellas, in the 1970s and 1980s, after selection on segregating material originating from International Maize and Wheat Improvement Center (CIMMYT). Seeds from each line were used to determine alleles at the loci for high molecular weight glutenin subunits (HMW-GS) Glu-A1, Glu-B1, Glu-R1 (or Sec-3 ) and gliadins (the loci Gli-A1 and Gli-B1 ). For this to be done, acid polyacrylamide gel electrophoresis for gliadins and SDS-electrophoresis for high molecular weight glutenins (HMW-GS) were applied. Analysis of the electrophoretic patterns obtained from the above-mentioned material revealed that only 5 out of the 11 cultivars were biochemically uniform (cv. ‘Vryto’, ‘Thisvi’, ‘Dada’, ‘Leto’ and ‘Ekate’). On the contrary, the rest of the cultivars, despite they were under seed production process, exhibited heterogeneity. Cv. ‘Dada’, which was found to be uniform, is of special interest, due to its productivity, especially under drought stress conditions.

Actions (login required)

Edit Item