Barley (Hordeum vulgare L.) marker linkage map: A case study of various marker types and of mapping population structure

Karsai, I. and Szűcs, P. and Mészáros, K. and Puskás, K. and Bedő, Z. and Veisz, O. (2007) Barley (Hordeum vulgare L.) marker linkage map: A case study of various marker types and of mapping population structure. Cereal Research Communications, 35 (4). pp. 1551-1562. ISSN 0133-3720

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Abstract

A barley mapping population consisting of 96 doubled haploid lines of anther culture origin was developed from the varieties Dicktoo and Kompolti Korai, which represent diverse types with respect to geographical origin and ecological adaptation. Several molecular marker techniques were used in mapping: among the markers with known chromosome location, RFLP, STS and SSR markers were applied to identify linkage groups and for comparative mapping, while RAPD and AFLP markers, which have random binding but provide useful information on polymorphism, were employed to fill in the linkage groups with markers. A total of 496 markers were tested in the DH population, 246 of which were included in the linkage map after eliminating markers that were completely linked with each other. The ratio of markers with known chromosome location to random markers in the 1107 cM map was one to three, and the mean recombination distance between the markers was 4.5 cM. Application of various marker methods and the effect of the population structure on the development of marker linkage maps are discussed.

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