Fluorescent in situ hybridization polymorphism on the 1RS chromosome arms of cultivated Secale cereale species

Szakács, É. and Molnár-Láng, M. (2008) Fluorescent in situ hybridization polymorphism on the 1RS chromosome arms of cultivated Secale cereale species. Cereal Research Communications, 36 (2). pp. 247-255. ISSN 0133-3720

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Abstract

The present study was focused on the selection of S. cereale cultivars of different geographic origin showing polymorphism detectable by fluorescent in situ hybridization on their 1RS chromosome arms. One perennial and four annual genotypes were tested. FISH was carried out with the DNA probes pSc119.2 and (AAC) ₅ . The pSc119.2 probe gave hybridization signals different from that of the rye ‘Petkus’ on the 1RS arms of all five rye cultivars examined. Differences were manifested mainly in the intensity of the labelling, but the complete lack of FISH signals and double signals were also observed.

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