The application of wheat microsatellite markers for the detection of interspecific variation in tetraploid Aegilops species with C and U genomes

Leonova, I. and Röder, M. and Nasyrova, F. (2009) The application of wheat microsatellite markers for the detection of interspecific variation in tetraploid Aegilops species with C and U genomes. Cereal Research Communications, 37 (3). pp. 335-343. ISSN 0133-3720

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Abstract

We report the application of wheat microsatellites (GWM) for the investigation of Aegilops species carrying C and Ugenomes. Overall, 89 microsatellite markers located in A, B and D genomes of bred wheat were used for the analysis of Ae. cylindrica Host. (CCDD) and Ae. triuncialis L. (UUCC). Ae. tauschii Coss. (DD) was included as a control of amplification of the D-genome markers. Twelve, eleven and seven of the A-genome-specific markers produced amplification fragments in Ae. cylindrica, Ae. triuncialis and Ae. tauschii , respectively. The level of amplification of the B-genome markers was similar in all investigated species and amounted to 60–65%. The markers of the D genome showed a significantly higher polymorphism level among Ae. tauschii accessions than among those of Ae. cylindrica . Twenty-one microsatellite markers revealing polymorphism in Ae. cylindrica and Ae. triuncialis can be used as a markers for discrimination of C and U genomes. The results of microsatellite analysis were used to estimate genetic relationships among the Aegilops species. The dendrogram distinguished all Aegilops accessions and clustered them into three groups according to their species classification. There was no strong relation between the molecular data of the studied accessions and their geographical region of origin. Obtained data could be utilized for characterization and assessing genetic diversity of wild wheat relatives with C and U genomes.

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