The nucleotide sequence of barley strain of Wheat dwarf virus isolated in Hungary

Tóbiás, I. and Kiss, B. and Salánki, K. and Palkovics, L. (2010) The nucleotide sequence of barley strain of Wheat dwarf virus isolated in Hungary. Cereal Research Communications, 38 (1). pp. 67-74. ISSN 0133-3720

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Abstract

Barley-infecting isolates of Wheat dwarf virus (WDV) were collected in the field in the vicinity of the cities Dunakiliti, Heves and Siófok, in Hungary. Viral genomic DNA was amplified by the rolling circle amplification technique, digested with Hind III, cloned into pBSK+ plasmid and sequenced. The clones were of the same size and showed above 99% identity to each other. Based on DNA sequences WDV-D01, WDV-H1 and WDV-H07 isolates showed high identity (94–99%) to isolates of WDV barley strain and Barley dwarf virus and lower identity to Oat dwarf virus (71% identity) and WDV wheat strains (85% identity).

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