Sequence polymorphism and expression analysis of genes encoding gibberellin 20-oxidase from Dasypyrus villosum

Cai, P. and Long, H. and Liang, J. and Wang, C. and Deng, G. and Pan, Z. and Peng, Z. and Yu, M. (2011) Sequence polymorphism and expression analysis of genes encoding gibberellin 20-oxidase from Dasypyrus villosum. Cereal Research Communications, 39 (3). pp. 365-375. ISSN 0133-3720

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Abstract

Based on the known GA20-oxidase (GA20ox) cDNAs of barley and wheat, oligonucleotide primers were designed to isolate GA20ox genes from genomic DNA of Dasypyrum villosum. A total of 19 clones were obtained. Each of them contained an open reading frame encoding a putative 40-KDa protein of 359 amino acid residues. Twenty-one SNPs and 4 InDels were found and could divide the 19 sequences into 2 classes, designated as DvGA20ox-1 and DvGA20ox-2, respectively. Q-PCR analyses showed that both DvGA20ox-1 and DvGA20ox-2 were in leaf blade, leaf sheath, stem, eustipes, root and developing spike. Similar expression levels were found between DvGA20ox-1 and DvGA20ox-2 in three stages. The total expression levels of DvGA20ox-1 and DvGA20ox-2 presented downtrend in leaf blade and ascend in stem, eustipes and developing spike along with the development of plants, respectively. However, they were firstly increased and then decreased in root from seeding stage to heading stage. These results revealed that the gene expression profile of DvGA20ox-1 and DvGA20ox-2 closely related to the growth and development of D. villosum.

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