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Genetics of sulphate assimilation in Schizosaccharomyces Pombe

Simonics, T. and Bánszky, Luca and Maráz, Anna (2002) Genetics of sulphate assimilation in Schizosaccharomyces Pombe. Acta Microbiologica et Immunologica Hungarica, 49 (2-3). pp. 279-283. ISSN 1217-8950

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Abstract

Sulphur plays an important role in yeasts, especially in the biosynthesis of methionine and cysteine. The inorganic sulphur source, sulphate, is taken up by the cells via the sulphate-permease(s). After its transport, it is activated and subsequently reduced to sulphide or serves as a donor for sulphurylation reactions. Selenate anion (SeO42-), which has the same metabolic pathway as sulphate, is toxic for the cells of Schizosaccharomyces pombe. We isolated selenate resistant mutants which cannot utilize sulphate, therefore they need organic sulphur source for growth. One of the selenate resistant mutants was successively transformed with S. pombe genomic libraries and the gene complementing the selenate resistance was identified as that of coding for the ATP-sulphurylase enzyme.

Item Type: Article
Subjects: Q Science / természettudomány > QR Microbiology / mikrobiológia
Depositing User: xFruzsina xPataki
Date Deposited: 11 Sep 2017 07:00
Last Modified: 11 Sep 2017 16:01
URI: http://real.mtak.hu/id/eprint/62165

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