Serotypes, virulence factors, antibiotic sensitivity, beta-lactamase activity and plasmid analysis of Salmonella from children with diarrhea in Tripoli (Libya)

El-Ghodban, A. and Ghenghesh, K. S. and Márialigeti, K. and Abeid, S. (2002) Serotypes, virulence factors, antibiotic sensitivity, beta-lactamase activity and plasmid analysis of Salmonella from children with diarrhea in Tripoli (Libya). Acta Microbiologica et Immunologica Hungarica, 49 (4). pp. 433-444. ISSN 1217-8950

Preview

Text amicr.49.2002.4.2.pdf Download (490kB) | Preview

Abstract

A total of 21 Salmonella strains isolated in Libya (16 from children with diarrhea and 5 from healthy controls) were serotyped and studied for their cell invasive ability, production of cytotoxin, antibiotic susceptibility, β-lactamase activity and plasmid profiles. Eight different serotypes of Salmonella were identified: 6 S. saintpaul , 4 S. wien (1 from control), 2 S. newport , 2 S. muenchen (1 from contr ol), 2 S. typhimurium (1 from control) , 2 S. hadar (1 from control), 2 S. reading (1 from control), 1 S. kottbus . Twenty (95%) were positive in the invasiveness assay using HeLa cells, and all (100%) were negative for cytotoxin production in HT29 cells. More than 40% were resistant to ampicillin, cefalexin, cefam andole, cefoperazone, chloramphenicol, gentamicin, mezlocillin and trimethoprim- sulphamethoxazole and 100% were susceptible to the new quinolones. Most (67%) of the strains harbored plasmids and 43% produced β-lactamase. A strong association was observed between the presence of more than one plasmid, β-lactamase activity, and multiple-resistance to antimicrobial agents and serotypes S. saintpa ul and S. wien . Curing experiments with acridine orange showed that 2 plasmids (33 and 1.4 megadaltons ) might be responsible for the resistance to chloramphenicol and gentamicin. The present study demonstrated that multiple-resistant salmonellae are widesp read in Libya and the resistance is mainly plasmid mediated.

Actions (login required)

Edit Item