REAL

Neuroprotective effects of repeated transient global ischemia and of kynurenine adminsitration induced by four-vessel occlusions on hippocampal CA1 neurons.

Toldi, József and Robotka, Hermina and Kopcsányi, Tamás and Sas, Katalin and Rákos, Gabriella and Kis, Zsolt and Farkas, Tamás and Marosi, Máté and Vécsei, László and Raceková, Eniko and Burda, Jozef (2007) Neuroprotective effects of repeated transient global ischemia and of kynurenine adminsitration induced by four-vessel occlusions on hippocampal CA1 neurons. In: Göttingen Meeting of the German Neuroscience Society 2007: 31st Göttingen Neurobiology Conference, 2007.03.29-2007.04.01, Göttingen, Németország.

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Abstract

The hippocampal CA1 subfield is a brain region that is particularly sensitive to hypoxia. Although this subfield is selectively vulnerable to ischemic injuries manifested in delayed neuronal death (DND), the mechanism leading to neuronal degeneration is not fully understood. Burda recently reported that a second pathophysiological stress, applied within a suitable time, offers an opportunity for salvaging neurons in the CA1 region against DND (Neurochem. Res., 30: 1397-1405, 2005). In our study, NeuN immunohistochemistry was applied to detect survival CA1 neurons, while Fluoro-Jade B staining was used to evaluate the number of injured neurons after interventions resulting in transient global ischemia. Four groups of animals were used: 1: intact controls; 2: sham controls (2 vertebral arteries coagulated (2VAC), but 2 carotids sham-operated); 3: 2VAC + 2 carotids occluded (2CA) for 10 min; 4: 2VAC + 2CA (10 min) + 2 days later, a repeated 2CA (5 min). In group 3 (2VAC + 2CA (10 min)), marked cell destruction was found in the CA1 subfield: only 36.4% of the CA1 neurons survived. However, in group 4 (5-min second ischemic insult), the proportion of surviving cells in the CA1 region was 59.3%. There was no significant difference in CA1 cell loss between groups 1 and 2. Our findings suggest that the second ischemic stress, 2 days after the first ischemia induced by 2VAC + 2CA can be efficient in the prevention of DND. Neuroprotective effect was also found in four-vessel occlusion models after kynurenine (i.v.) administration.

Item Type: Conference or Workshop Item (Paper)
Subjects: R Medicine / orvostudomány > RC Internal medicine / belgyógyászat > RC0321 Neuroscience. Biological psychiatry. Neuropsychiatry / idegkórtan, neurológia, pszichiátria
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 08 Aug 2013 14:21
Last Modified: 08 Aug 2013 14:21
URI: http://real.mtak.hu/id/eprint/6221

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