Enzymatic synthesis of isopropyl myristate using immobilized lipase from Bacillus cereus MTCC 8372

Verma, M. L. and Chauhan, G. S. and Kanwar, S. S. (2008) Enzymatic synthesis of isopropyl myristate using immobilized lipase from Bacillus cereus MTCC 8372. Acta Microbiologica et Immunologica Hungarica, 55 (3). pp. 327-342. ISSN 1217-8950

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Abstract

A purified alkaline thermo-tolerant bacterial lipase from Bacillus cereus MTCC 8372 was immobilized on a Poly (MAc- co -DMA- cl -MBAm) hydrogel. The hydrogel showed approximately 94% binding capacity for lipase. The immobilized lipase (2.36 IU) was used to achieve esterification of myristic acid and isopropanol in n -heptane at 65 °C under continuous shaking. The myristic acid and isopropanol when used at a concentration of 100 mM each in n -heptane resulted in formation of isopropyl myristate (66.0 ± 0.3 mM) in 15 h. The reaction temperature below or higher than 65°C markedly reduced the formation of isopropyl myristate. Addition of a molecular sieve (3 Å × 1.5 mm) to the reaction mixture drastically reduced the ester formation. The hydrogel bound lipase when repetitively used to perform esterification under optimized conditions resulted in 38.0 ± 0.2 mM isopropyl myristate after the 3 ^rd cycle of esterification.

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