Cytochrome CYP141: A new target for direct detection of Mycobacterium tuberculosis from clinical specimens

Darban-Sarokhalil, Davood and Fooladi, Abbas Ali Imani and Bameri, Zakaria and Nasiri, Mohammad Javad and Feizabadi, Mohammad Mehdi (2011) Cytochrome CYP141: A new target for direct detection of Mycobacterium tuberculosis from clinical specimens. Acta Microbiologica et Immunologica Hungarica, 58 (3). pp. 211-217. ISSN 1217-8950

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Abstract

Cytochrome P450 CYP141 is an intermediary metabolic and respiratory protein that interferes with oxidation reduction in Mycobacterium tuberculosis. This conserved protein has also been debated as a hypothetical target for therapeutics. We used the sequences of CYP141 gene to develop a PCR for rapid detection of Mycobacterium tuberculosis from respiratory specimens. The sensitivity of this PCR for culture positive-smear positive and culture positive-smear negative samples were 92% and 62.5%, respectively. The overall sensitivity and specificity of this PCR was 85.7% and 97.8%. As compared with other studies, it appears that the CYP141 gene is a good target for direct detection of M. tuberculosis from respiratory specimens.

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