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Fast 3D Imaging of Spine, Dendritic, and Neuronal Assemblies in Behaving Animals

Szalay, Gergely and Judák, Linda and Katona, Gergely and Ócsai, Katalin and Juhász, Gábor and Veress, Máté and Szadai, Zoltán and Fehér, András and Tompa, Tamás and Chiovini, Balázs and Maák, Pál and Rózsa, Balázs (2016) Fast 3D Imaging of Spine, Dendritic, and Neuronal Assemblies in Behaving Animals. NEURON, 92 (4). pp. 723-738. ISSN 0896-6273

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Abstract

Understanding neural computation requires methods such as 3D acousto-optical (AO) scanning that can simultaneously read out neural activity on both the somatic and dendritic scales. AO point scanning can increase measurement speed and signal-tonoise ratio (SNR) by several orders of magnitude, but high optical resolution requires long point-topoint switching time, which limits imaging capability. Here we present a novel technology, 3D DRIFT AO scanning, which can extend each scanning point to small 3D lines, surfaces, or volume elements for flexible and fast imaging of complex structures simultaneously in multiple locations. Our method was demonstrated by fast 3D recording of over 150 dendritic spines with 3D lines, over 100 somata with squares and cubes, or multiple spiny dendritic segments with surface and volume elements, including in behaving animals. Finally, a 4-fold improvement in total excitation efficiency resulted in about 500 3 500 3 650 mm scanning volume with genetically encoded calcium indicators (GECIs).

Item Type: Article
Subjects: R Medicine / orvostudomány > R1 Medicine (General) / orvostudomány általában
R Medicine / orvostudomány > R1 Medicine (General) / orvostudomány általában > R850-854 Experimental medicine / kisérleti orvostudomány
Depositing User: Dr. József Balázs Rózsa
Date Deposited: 28 Sep 2017 22:00
Last Modified: 28 Sep 2017 22:00
URI: http://real.mtak.hu/id/eprint/64218

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