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The slow sarco/endoplasmic reticulum Ca2+-ATPase declines independently of slow myosin in soleus muscle of diabetic rats

Rácz, Gábor and Szabó, András and Vér, Ágota and Zádor, Ernő (2009) The slow sarco/endoplasmic reticulum Ca2+-ATPase declines independently of slow myosin in soleus muscle of diabetic rats. ACTA BIOCHIMICA POLONICA, 56 (3). pp. 487-493. ISSN 0001-527X

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Abstract

The sarcoplasmic reticulum Ca2+-ATPase (SERCA) isoforms are normally expressed in coordination with the corresponding myosin heavy chain (MyHC) isoforms in the fibers of skeletal muscle but this coordination is often disrupted in pathological conditions. In the streptozotocin-induced diabetes of rats (stz-rats), the soleus muscle showed peripheral neuropathy and the SERCA2a level decreased in type I (slow-oxidative) fibers compared to the control muscles, whereas the expression of the corresponding slow MyHC1 did not change. No difference was found at the mRNA and protein levels of SERCA and MyHC isoforms in the whole soleus, except that the level of the SERCA2a protein specifically declined in stz-rats compared to the controls. This shows that the coordinated expression of SERCA2a and MyHC1 is disrupted at the SERCA2a protein level in the diabetic soleus. The results are in line with previous observations that regulators of the Ca-homeostasis may adapt faster to type I diabetes than the contractile elements.

Item Type: Article
Uncontrolled Keywords: Sarcoplasmic Reticulum Calcium-Transporting ATPases; Reverse Transcriptase Polymerase Chain Reaction; Rats, Wistar; RATS; PROTEIN ISOFORMS; Myosin Heavy Chains; Muscle, Skeletal; Male; IMMUNOHISTOCHEMISTRY; immunoblotting; Diabetes Mellitus, Experimental; Animals; Wistar rat; skeletal muscle; reverse transcription polymerase chain reaction; RAT; METABOLISM; GENETICS; experimental diabetes mellitus; enzymology; ARTICLE; animal; sarcoplasmic reticulum calcium transporting adenosine triphosphatase; myosin heavy chain; isoprotein; Rattus; SERCA; MYOSIN; Muscle Fibers; diabetic rat; Sarcoplasmic Reticulum Calcium-Transporting ATPases/genetics/*metabolism; Protein Isoforms/genetics/metabolism; Myosin Heavy Chains/genetics/*metabolism; Muscle, Skeletal/*enzymology/*metabolism; Diabetes Mellitus, Experimental/*metabolism
Subjects: Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3011 Biochemistry / biokémia
Q Science / természettudomány > QH Natural history / természetrajz > QH301 Biology / biológia > QH3015 Molecular biology / molekuláris biológia
R Medicine / orvostudomány > R1 Medicine (General) / orvostudomány általában
R Medicine / orvostudomány > RB Pathology / patológia, kórtan
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 06 Sep 2013 09:57
Last Modified: 09 Sep 2013 08:37
URI: http://real.mtak.hu/id/eprint/6481

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