REAL

The influence of laparoscopic and open surgery on the concentration and structural modifications of insulin-like growth factor binding protein 3 in the human circulation

Baričević, I. and Malenković, V. and Jones, D.R. and Nedić, O. (2006) The influence of laparoscopic and open surgery on the concentration and structural modifications of insulin-like growth factor binding protein 3 in the human circulation. Acta Physiologica Hungarica, 93 (4). pp. 361-369. ISSN 0231-424X

[img] Text
aphysiol.93.2006.4.12.pdf
Restricted to Repository staff only until 31 December 2026.

Download (957kB)

Abstract

Insulin -like growth factor binding protein 3 (IGFBP -3) is the most abundant insulin -like growth factor binding protein in the circulation. The aim of the present work was to investigate the influence of surgery (laparoscopy and open) on the concentration an d carbohydrate content of IGFBP -3. The concentration of IGFBP -3 was measured using an immunoradiometric assay (IRMA), its protein profile was characterised using immunoblotting and its sialic acid content was examined by means of Sambucus nigra agglutinin (SNA) affinity chromatography. The concentration of IGFBP -3 was significantly (p<0.001) lower in preoperative patients compared with healthy subjects. Only patients that underwent open surgery showed a further significant decrease in the concentration of IGFBP -3. Immunoblotting detected two intact IGFBP -3 isoforms, as well as proteolytic fragments. SNA -affinity chromatography showed that in patients that underwent surgery the ratio between the two IGFBP -3 glycoforms was lower than the ratio in healthy sub jects. Patients with gallbladder inflammation that underwent laparoscopy had an increased percentage of specifically bound IGFBP -3 to SNA compared with healthy subjects. Our conclusion is that open surgery decreased the level of IGFBP -3 compared with lapar oscopy, whereas patients with gallbladder inflammation that underwent laparoscopy had an increased content of sialic acid in IGFBP -3.

Item Type: Article
Subjects: R Medicine / orvostudomány > R1 Medicine (General) / orvostudomány általában
Depositing User: xFruzsina xPataki
Date Deposited: 13 Nov 2017 07:27
Last Modified: 13 Nov 2017 07:27
URI: http://real.mtak.hu/id/eprint/65194

Actions (login required)

Edit Item Edit Item