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Morphology and Nanomechanics of Sensory Neurons Growth Cones following Peripheral Nerve Injury

Martin, Marta and Benzina, Ouafa and Szabo, Vivien and Végh, Attila Gergely and Lucas, Olivier and Cloitre, Thierry and Scamps, Frédérique and Gergely, Csilla (2013) Morphology and Nanomechanics of Sensory Neurons Growth Cones following Peripheral Nerve Injury. PLOS ONE, 8 (2). ISSN 1932-6203

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Abstract

A prior peripheral nerve injury in vivo, promotes a rapid elongated mode of sensory neurons neurite regrowth in vitro. This in vitro model of conditioned axotomy allows analysis of the cellular and molecular mechanisms leading to an improved neurite re-growth. Our differential interference contrast microscopy and immunocytochemistry results show that conditioned axotomy, induced by sciatic nerve injury, did not increase somatic size of adult lumbar sensory neurons from mice dorsal root ganglia sensory neurons but promoted the appearance of larger neurites and growth cones. Using atomic force microscopy on live neurons, we investigated whether membrane mechanical properties of growth cones of axotomized neurons were modified following sciatic nerve injury. Our data revealed that neurons having a regenerative growth were characterized by softer growth cones, compared to control neurons. The increase of the growth cone membrane elasticity suggests a modification in the ratio and the inner framework of the main structural proteins.

Item Type: Article
Subjects: R Medicine / orvostudomány > R1 Medicine (General) / orvostudomány általában
SWORD Depositor: MTMT SWORD
Depositing User: MTMT SWORD
Date Deposited: 15 Oct 2013 12:10
Last Modified: 15 Oct 2013 12:10
URI: http://real.mtak.hu/id/eprint/6886

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