In vitrostudy of AMP-deaminase from fish (Cyprinus carpio)treated with hydrolyzable tannins

Pałecz, Danuta and Jaszczuk, Ewa and Gabryelak, Teresa (2006) In vitrostudy of AMP-deaminase from fish (Cyprinus carpio)treated with hydrolyzable tannins. Acta Biologica Hungarica, 57 (1). pp. 49-56. ISSN 0236-5383

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Abstract

AMP-deaminase (EC 3.5.4.6) is an enzyme responsible for stabilising adenylate energy changes. The properties of this enzyme are controlled by various ligands of hydrophobic nature. An investigation of enzyme activity alterations under the influence of natural phenolic acids (tannic, ellagic and gallic) which are soluble in water, could evidence the biological toxicity of these compounds. In our study purified AMP-deaminase isolated from white muscle of Cyprinus carpio was exposed to phenolic acids in the concentration range of 1 to 50 μM as well as to tannic acid in the presence of Cu 2+ ions (5 μM). On the basis of the obtained results we can conclude that among the tested acids, gallic acid did not contribute to the change in AMP-deaminase activity, whereas ellagic acid diminished its activity at the highest con- centration (50 μM). Tannic acid caused a significant decrease in the enzyme activity in comparison to control for all used concentrations. Cu 2+ ions alone reduced the activity of AMP-deaminase for all stud- ied concentrations. A combined action of a chosen Cu 2+ ions concentration (5 μM) with tannic acid at the concentration higher than 2 μM resulted in a decrease in the enzyme activity, but for lower tannic acid concentration of 1 μM the activity of AMP-deaminase was stimulated. These experiments showed that tannic acid may stop free radical chain reactions only at low concentrations (1 μM) in the presence of Cu 2+ ions (5 μM).

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