Amplified Fragment Length Polymorphism (AFLP) is useful for finding markers associated with QTL for architectural trait in Hedysarum coronarium L.

Marghali, S. and Chennaoui, H. and Bourguiba, H. and Marrakchi, M. and Trifi-Farah, N. (2006) Amplified Fragment Length Polymorphism (AFLP) is useful for finding markers associated with QTL for architectural trait in Hedysarum coronarium L. Acta Biologica Hungarica, 57 (4). pp. 459-471. ISSN 0236-5383

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Abstract

Amplified fragment length polymorphism (AFLP) has been proposed as a valuable tool for finding mol- ecular markers linked to QTL controlling architectural trait. Segregation of polymorphic AFLP frag- ments was followed in F 2 offsprings Hedysarum coronarium derived from two native wild accesions crossing: Jebel Zit × El Haouaria, which had previously characterised by morphological and AFLP mark- ers. A comparison of profiles from the morphologically contrasted parental individuals and F 2 offspring has been assessed using five AFLP primer combinations. These generated 178 bands revealed by silver- stained denaturing polyacrylamide gels, of which 150 were polymorphic. Several AFLP markers appeared to be implied in the orthotropic form that can assist the selection and improve Hedysarum for- age crop.

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