REAL

Somatic embryogenesis in apomict Boechera holboellii

Taskin, K. and Turgut, K. and Scott, R. (2009) Somatic embryogenesis in apomict Boechera holboellii. Acta Biologica Hungarica, 60 (3). pp. 301-307. ISSN 0236-5383

[img] Text
abiol.60.2009.3.7.pdf
Restricted to Repository staff only until 30 September 2029.

Download (1MB)

Abstract

In this study we establish an efficient method for the regeneration for Boechera holboellii via somatic embryogenesis. Immature cotyledons from siliques of 4–6 month-old plants were cultured on MS medium supplemented with plant growth regulators (BA — 6-benzylaminopurine; NAA — α-naphthaleneacetic acid; TDZ — 1-phelyl-3-(1,2,3-thiadiazol-5-yl) urea: 2,4 D-2,4-dichlorophenoxy-acetic acid). A high frequency of embryogenic callus was produced after two weeks in culture. The somatic embryos were obtained with a frequency of 10% of explants on MS medium supplemented with 1.34 μM NAA + 8.87 μM BA and 2.68 μM NAA + 17.74 μM BA within 3 weeks in culture. The alternative regime of MS medium supplemented with 1.34 μM NAA + 4.44 μM BA produced somatic embryos at a frequency of 38%.

Item Type: Article
Subjects: Q Science / természettudomány > QH Natural history / természetrajz
Depositing User: xEndre xSarvay
Date Deposited: 23 Nov 2017 14:49
Last Modified: 23 Nov 2017 14:49
URI: http://real.mtak.hu/id/eprint/70348

Actions (login required)

Edit Item Edit Item