REAL

Energy production and redox status of rat red blood cells after reticulocytosis induced by various treatments

Marković, Snežana and Žižić, Jovana and Obradović, Ana and Ognjanović, Branka and Štajn, A. and Saičić, Zorica and Spasić, M. (2011) Energy production and redox status of rat red blood cells after reticulocytosis induced by various treatments. Acta Biologica Hungarica, 62 (2). pp. 122-132. ISSN 0236-5383

[img] Text
abiol.62.2011.2.2.pdf
Restricted to Repository staff only until 30 June 2031.

Download (290kB)

Abstract

Stimulated erythropoiesis and reticulocytosis can be induced by daily bleeding, or by phenylhydrazine (PHZ) treatment. We compared the in vivo effects of PHZ and bleeding treatment on haematological, energy and redox status parameters in red blood cells (RBC) of rats. The results showed that all followed haematological parameters were significantly lower in bleeding, compared to PHZ-treated rats. PHZ induced even 2.58-fold higher reticulocytosis as compared to bleeding treatment. Although PHZ induced higher reticulocytosis, respiration intensity and energy production was lower than in bleeding-induced reticulocytes. These alterations were the consequence of increased superoxide anion and peroxynitrite concentrations in PHZ-treated rats. Bleeding treatment resulted in increased activity of an antioxidative enzyme, superoxide dismutase. In conclusion, differences in these two experimental models for reticulocytosis may be used as tools for appropriate pharmacological testing of redox-active substances considering energy and redox processes, as well as apoptosis pathways.

Item Type: Article
Subjects: Q Science / természettudomány > QH Natural history / természetrajz
Depositing User: Endre Sarvay
Date Deposited: 23 Nov 2017 16:23
Last Modified: 23 Nov 2017 16:23
URI: http://real.mtak.hu/id/eprint/70444

Actions (login required)

Edit Item Edit Item