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Bioassay-comparison of the antioxidant efficacy of hydrogen sulfide and superoxide dismutase in isolated arteries and veins

Hamar, J. and Solymár, M. and Tanai, E. and Cseplo, P. and Springo, Zs. and Berta, G. and Debreceni, B. and Koller, Akos (2012) Bioassay-comparison of the antioxidant efficacy of hydrogen sulfide and superoxide dismutase in isolated arteries and veins. Acta Physiologica Hungarica, 99 (4). pp. 411-419. ISSN 0231-424X

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Abstract

Recent studies suggest that hydrogen sulfide (H2S) exhibits potent antioxidant capacity and improves vascular and tissue functions. Thus we aimed to compare the antioxidant efficacy of H2S to that of superoxide dismutase (SOD).Isometric force of isolated rat carotid arteries and gracilis veins was measured with a myograph. The vasomotor effect of the superoxide-generator pyrogallol (10−5M) was obtained in control conditions, and then in the presence of SOD (120 U/ml) or H2S (10−5M or 10−4M), respectively. Spectrophotometric measurements were performed to detect the effect of SOD and H2S on the auto-oxidation of pyrogallol.Pyrogallol increased the isometric force of carotid arteries (9.7 ± 0.8 mN), which was abolished by SOD (5.3 ± 0.8 mN), was not affected by 10−5M H2S (9.1 ± 0.5 mN), whereas 10−4M H2S slightly, but significantly reduced it (8.1 ± 0.7 mN). Pyrogallol significantly increased the isometric force of gracilis veins (1.3 ± 0.2 mN), which was abolished by SOD (0.9 ± 0.2 mN), whereas 10−5M (1.3 ± 0.2 mN), or 10−4M H2S (1.2 ± 0.2 mN) did not affect it. Pyrogallol-induced superoxide production was measured by a spectrophotometer (A420 = 0.19 ± 0.0). SOD reduced absorbance (A420 = 0.02 ± 0.0), whereas 10−5M H2S did not (A420 = 0.18 ± 0.0) and 10−4M H2S slightly reduced it (A420 = 0.15 ± 0.0).These data suggest that H2S is a less effective vascular antioxidant than SOD. We propose that the previously described beneficial effects of H2S are unlikely to be related to its direct effect on superoxide.

Item Type: Article
Subjects: R Medicine / orvostudomány > R1 Medicine (General) / orvostudomány általában
Depositing User: xFruzsina xPataki
Date Deposited: 24 Nov 2017 08:08
Last Modified: 24 Nov 2017 08:08
URI: http://real.mtak.hu/id/eprint/70531

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