The effect of dietary immunostimulants on the susceptibility of common carp (Cyprinus carpio) to the white spot parasite, Ichthyophthirius multifiliis

Herczeg, Dávid and Sipos, Dóra and Dán, Ádám and Loy, Christina and Kallert, Dennis M. and Eszterbauer, Edit (2017) The effect of dietary immunostimulants on the susceptibility of common carp (Cyprinus carpio) to the white spot parasite, Ichthyophthirius multifiliis. Acta Veterinaria Hungarica, 65 (4). pp. 517-530. ISSN 0236-6290

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Abstract

One of the main obstacles in freshwater aquaculture is the parasitic ciliate Ichthyophthirius multifiliis (Ich), the causative agent of white spot disease. The use of immunostimulants as feed additives may be a promising approach to control Ich infection. In the present study, we tested the prophylactic effect of orally administered β-1,3/1,6-glucan and propolis extract E50 against Ich infection in common carp. In total, 122 fish were separated into three experimental groups fed with a control, 3% β-glucan and 1% propolis diet for 40 consecutive days, respectively. On day 40, 16 fish per group were individually exposed to Ich theronts and the number of trophonts was counted 5 days post exposure. Relative gene expression of interleukin 1-β (IL-1-β) in common carp liver was examined by qPCR. Compared to control, the mean infection intensity was lower in the β-glucan- and propolis-fed groups; however, the difference was not statistically significant. The relative expression of IL-1-β significantly decreased in the propolis-fed group at day 10. In the β-glucan-fed group, a significant IL-1-β decrease was detected at day 15 compared to control. Although the Ich infection intensity was slightly decreased in both treated groups, and IL-1-β was moderately down-regulated in the liver of common carp, our results suggest that the applied feeding regime is insufficient to prevent Ich outbreaks in common carp.

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