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Isolation and characterization of an atypical Siberian sturgeon herpesvirus (SbSHV) strain in Russia: novel North-American Acipenserid herpesvirus 2 strain in Europe?

Doszpoly, Andor and Kalabekov, Ismail M. and Breyta, Rachel and Shchelkunov, Igor S. (2017) Isolation and characterization of an atypical Siberian sturgeon herpesvirus (SbSHV) strain in Russia: novel North-American Acipenserid herpesvirus 2 strain in Europe? Journal of Fish Diseases, 40 (10). pp. 1363-1372. ISSN 0140-7775 (print), 1365-2761 (online)

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Abstract

Siberian sturgeon herpesvirus (SbSHV) was isolated in Russia for the first time in 2006. Nine SbSHV isolates were recovered from different fish hatcheries producing the same CPE in cell cultures, the same clinical signs and mortality kinetics in virus-infected fish, the same virus neutralization pattern, and shared identical nucleotide sequences. In 2011 a new isolate was recovered from juvenile sturgeon, which caused completely different CPE. That isolate was not readily neutralized by Siberian sturgeon hyperimmune antisera and its DNA was not recognized by the routine PCR developed for SbSHV detection. Molecular study of the novel isolate revealed that it was more closely related to North-American Acipenserid herpesvirus 2 (AciHV-2) isolates from white sturgeon, while the genome sequences of the former SbSHV isolates showed high similarity to the AciHV-2 isolated from shortnose sturgeon. While clinical signs and mortality caused by the novel isolate in infected Siberian sturgeon were similar to those of the formerly described SbSHV isolates, the incubation period and mean time to death produced by the novel isolate were twice as long. The differences between the former isolates and the recent one suggest that a novel SbSHV strain emerged in Europe and the molecular findings imply its North-American origin.

Item Type: Article
Subjects: Q Science / természettudomány > QR Microbiology / mikrobiológia > QR355 Virology / víruskutatás
Depositing User: Dr Andor Doszpoly
Date Deposited: 08 Jan 2018 12:40
Last Modified: 08 Jan 2018 12:40
URI: http://real.mtak.hu/id/eprint/72159

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