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The optimization of Regeneration tissue culture system of three chilli peppers cultivars based on the uniform design and the mathematical model equation

Hu, Tingzhang and Zeng, Hua and Chen, Zaigang and Huang, Xiaoyun and Yang, Yongwei and Wang, Guixue (2012) The optimization of Regeneration tissue culture system of three chilli peppers cultivars based on the uniform design and the mathematical model equation. Acta Biologica Hungarica, 63 (3). pp. 372-388. ISSN 0236-5383

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Abstract

Using uniform random design optimization and the mathematical model equation we optimized the regeneration tissue culture system of the chilli pepper. An efficient and detailed plant reproducible protocol in vitro has been established using different explants and induction media for three chilli pepper cultivars. The result displayed that the seedlings at the curved hypocotyl stage were the best choice to prepare for explants, the genotype of explants affected shoot buds induction frequency and number of shoot buds per explant, and the cotyledon explant was more responsive than hypocotyl explant. The optimal media for maximum shoot initiation and regeneration and the optimal elongation medium were obtained. For Capsicum annuum var. annuum (cv. Xinsu), Capsicum annuum var. annuum (cv. Neimengchifeng) and Capsicum frutescens (cv. Xingfu), the induction rates were 99.17%, 97.50 and 96.11%, respectively; the elongation rates of shoot buds were 86.67%, 85.19% and 82.96%, respectively. The MS medium with 0.57 μM IAA and 0.69 μM NAA is the best choice for root induction. The frequency of their root emergence was 95.00–98.33%. Regenerated chilli peppers were successfully acclimatized and cultivated with 100% survival. This work will help to improve multiplication process and the genotype of chilli pepper, and may have commercial impact.

Item Type: Article
Subjects: Q Science / természettudomány > QH Natural history / természetrajz
Depositing User: Ágnes Sallai
Date Deposited: 12 Jan 2018 07:33
Last Modified: 12 Jan 2018 07:33
URI: http://real.mtak.hu/id/eprint/72381

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